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Northern blot analysis of differential signals in the screening. Each lane contains 20 μg of total RNA from M. tuberculosis-infected U937 cells and M. marinum-infected U937 cells/ml. The blot was hybridized with the IL-8 probe used for Northern analysis. A higher IL-8 expression level was observed in M. tuberculosis-infected cells. Loading equality was demonstrated using a β-actin probe (bottom).
