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Induction of IκBα in mycobacterium-infected U937 cells. Cytoplasmic extracts were assessed for IkBα activity by Western blot analysis. Protein from equal numbers of cells was loaded in each lane. (A) Kinetic analysis of IkBα activity in U937 cells infected with M. tuberculosis H37Rv. (B) Activation of IkBα by U937 cells infected with M. tuberculosis H37Rv and M. marinum. The MOI was 10.
