FIG.4.

Localization of GFP-Rab GTPases in C. pneumoniae AR39-infected HeLa cells. HeLa cells were transiently transfected with DNA containing the indicated GFP-tagged Rab GTPases (B to L) or pEGFPC1 (A). At 24 h posttransfection, the cells were infected with C. pneumoniae strain AR39, and at 44 h postinfection, the cells were fixed and viewed by LSCM. Arrowheads indicate chlamydial inclusions, as determined by indirect immunofluorescence staining with antichlamydial LPS (data not shown). Specific associations between GFP-Rab1A, GFP-Rab4 (GFP-Rab4A and GFP-Rab4B), GFP-Rab10, and GFP-Rab11 (GFP-Rab11A and GFP-Rab11B) and the C. pneumoniae inclusion were detected, as shown by rim-like fluorescence staining around the entire periphery of the inclusion. Bar, 10 μm.