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. 2003 Oct;71(10):5855–5870. doi: 10.1128/IAI.71.10.5855-5870.2003

FIG. 7.

FIG. 7.

GFP-Rab11A partially colocalizes with Tfn-containing endosomes at the periphery of the chlamydial inclusion. Transiently transfected HeLa cells expressing GFP-Rab11A were infected with C. trachomatis serovar L2 for 18 h. Recycling endosomes were labeled with Alexa 568-labeled Tfn by serum starving cells for 2 h and incubated with Alexa 568-labeled Tfn for 60 min at 37°C. Cells were fixed, permeabilized, stained with antichlamydial LPS, and incubated with goat anti-mouse IgG conjugated to Cy5 (blue). In the inset in panel D, the arrowhead indicates Rab11A-positive Tfn-negative regions around the inclusion and the arrow indicates Rab11A-positive Tfn-positive regions. (A) GFP-Rab11A (green), (B) Alexa 568-labeled Tfn (red), (C) antichlamydial LPS (blue), (D) panels A, B, and C merged. Bar, 5 μm.