FIG. 2.
Western blot analysis of whole-cell lysates of strain P833. Serum from individual subjects (FS1 and FS11) colonized with the serotype 23F isolate (P833) were used to visualize the IgG response during carriage to PspA or CbpA (as indicated by arrows). Lanes: 1, FS1 preinoculation serum; 2, FS1 serum from 2 weeks after the last detected colonization; 3, FS1 serum from 2 weeks after the last detected colonization preincubated with 1 μg of recombinant P833-derived PspA31-189/ml; 4, FS11 preinoculation serum; 5, FS11 serum from 2 weeks after the last detected colonization; 6, FS11 serum from 2 weeks after the last detected colonization preincubated with 10 μg of P833-derived recombinant CbpA/ml.