TABLE 1.
Primers used to generate recombinant His-tagged proteins for use in ELISAsa
| Construct | 5′ primer sequence | 3′ primer sequence |
|---|---|---|
| PspA31-87 | GGAATTCCATATGGAAGAAGCTCCC | CCGGAATTCTCTCAGCTTCTTCTG |
| PspA31-162 | GGAATTCCATATGGAAGAAGCTCCC | GGAATTCTAAGGAACTACTTTTGCTTGTTCC |
| PspA87-162 | GGGTTTCCATATGACTGAAGAGAAAGCAAAGCTT | GGAATTCTAAGGAACTACTTTTGCTTGTTCC |
| PspA202-274 | AAAGCTGTTGAAGCCAAACAA | ACTCAACTCTTCAAGTTTTGATAG |
| PspA183-627 | GGGTTTCCATATGGAACCAGAACTTGCTAAAAAGTAG | GGAATTCGCGCGTCGACGGCTTAAACCCATTCACCATTGG |
| PpmA22-313 | CCATGGCTAGCCACCATCACCATCACCATTCGAAAGGGTCAGAAGGTGC | TCATGGATCCGGACTATTCGTTTGATGTAC |
| IgA1 protease671-1987 | CAACATCACATATGGGACAAACAGAACCAGAG | ACTTAGATCTTAATGATGGTGGTGATGATGGGCTTTAAAGATTGCTCTC |
| CbpA (P833) | AGTGTAGTAGTTGCTAGTCTTGTT | GGAATTCCCACATACCGTTTCTTGTTTC |
a
Primers are listed 5′→3′.