TABLE 1.

Oligonucleotide primers used in this study

Primers	Sequencea
lacI 5′	gggggagctcCGTTATTTCTTGATGTCTCTGAC
lacI 3′	ggggatccGCCTGGGGTGCCTAATGAGTGAG
lacA 5′	ccggatccAATGACCGAAAGAATAAGAG
lacA 3′	aaaactgcagATGTCTTTTGTGACGATACT
lacZ 5′	ccggatccggtaccATGACCATGATTACGGATTCAC
lacZ 3′	ccagatctCCTTACGCGAAATACGGGC
pSepL 5′	cgggatccGATTGAGGCCTTGTTCAAGG
pSepL 3′	cgggtaccTTCAATACCATTAGCCATTGG
lacI up 5′	CGCGGTATGGCATGATAGCG
lacA down 3′	CATGCCGGATGCGGCTAATG
lac mid 5′	GTGACGTCTGCTTGCTGCAT
lac mid 3′	CAGCAGGATATCCTGCACC
espA short 5′	ccggatccCGATTGTCGAAGATAAAC
espA short 3′	cggtgaccTGCATTTGATGTATCCAT
pgi probe 5′	ACATCAATCCAACGCAGACC
pgi probe 3′	TTCATCTTCTCCAGCACCGC
espA probe 5′	ACATCCGTTGTTAATGTGAGTGCG
espA probe 3′	GCAATTTTGGCATCCACAAG
sepL probe 5′	ATCAAAACCCCGCATCTGTT
sepL probe 3′	TTTCCTTGCGCTACCTTTGC
espD probe 5′	GCTTAACGTAAATAACGATACCCTG
espD probe 3′	CTCACCACTAATACCAAACAAT
escC probe 5′	ATTCGCTAGATGCAGATTTTATCGG
escC probe 3′	GTTACTTGATATTCAGGATGGC
lee1 5′	ccggatccCTGTAACTCGAATTAAGTAGAG
lee1 3′	ccggtaccGTATGGACTTGTTGTATGTGAATT
lee2 5′	cgggatccGCGAACGCGCTCAATAATCTG
lee2 3′	cgggtaccTGCTGCTTCCATTGATCTTTC
lee3 5′	cgggatccCTGCTCGTCTCCGAGCATGCC
lee3 3′	cgggtaccAACTAAAAGATTCATCTGCAGGCT
GFP 5′	ccggatccggggtaccATGGTGAGCAAGGGCGAGGAGC
GFP 3′	cgggtaccAACTAAAAGATTCATCTGCAGGCT

^aThe lowercase portions of some sequences are nonmatching sequence incorporating a restriction enzyme site.