TABLE 2.
Plasmids used in the study
| Plasmid(s) | Description |
|---|---|
| pIB307 | pMAK705-based vector for allelic exchange; temperature-sensitive replicon (4) |
| pDG005 | Low-copy-number vector containing MG1655 lacZYA region |
| pDG028 | Low-copy-number vector containing sac-kan cassette (21) |
| pACYC184 | Low-copy-number cloning vector |
| pAJR26 | lacIA flanking regions from ZAP193 cloned into pIB307 |
| pAJR27 | lacIA flanking regions from ZAP1 cloned into pIB307 |
| pAJR33 | pAJR26 with sac-kan cassette from pDG28 cloned between lacIA fragments |
| pAJR34 | pAJR27 with sac-kan cassette from pDG28 cloned between lacIA fragments |
| pAJR39 | pAJR26 with promoterless lacZ gene cloned between lacIA fragments |
| pAJR43 to 48 | pAJR39 with sepL promoter region from described ZAP strain cloned in frame of lacZ (pAJR43, ZAP1; pAJR44, ZAP21; pAJR45, ZAP41; pAJR46, ZAP46; pAJR47, ZAP58; pAJR48, ZAP193) |
| pAJR55 to 60 | pAJR39 with espA promoter region from described ZAP strain cloned in frame of lacZ (pAJR55, ZAP1; pAJR56, ZAP21; pAJR57, ZAP41; pAJR58, ZAP46; pAJR59, ZAP58; pAJR60, ZAP193) |
| peGFP | Clontech commercial vector |
| pAJR70 | pACYC184 cut with BamHI, gfp gene cloned BamHI/BglII |
| pAJR71 | pAJR70 cut with BamHI/KpnI; LEE1 promoter cloned in frame 5′ to gfp |
| pAJR72 | pAJR70 cut with BamHI/KpnI; LEE2 promoter cloned in frame 5′ to gfp |
| pAJR73 | pAJR70 cut with BamHI/KpnI; LEE3 promoter cloned in frame 5′ to gfp |
| pAJR74 | pAJR70 cut with BamHI/KpnI; LEE4 promoter cloned in frame 5′ to gfp |
| pAJR100 | pACYC184 cut with BamHI; lacZ gene cloned BamHI/Bgl/II |
| pAJR101 | pAJR100 cut with BamHI/KpnI; LEE1 promoter cloned in frame 5′ to lacZ |
| pAJR102 | pAJR100 cut with BamHI/KpnI; LEE2 promoter cloned in frame 5′ to lacZ |
| pAJR103 | pAJR100 cut with BamHI/KpnI; LEE3 promoter cloned in frame 5′ to lacZ |
| pJTPr-2 | pCB267 with LEE4 (sepL) promoter cloned 5′ to lacZ |