TABLE 3.
Strains used in the study
| Strain | Details | Source, reference |
|---|---|---|
| ZAP1 | O157:H7 Redhouse Dairy human-outbreak strain phage type 2 | Our stocks, 51 |
| ZAP21 | Bovine diarrhea strain STEC 413/89-1 | Our stocks, 13 |
| ZAP41 | O157:H7 bovine strain | Our stocks, 40 |
| ZAP46 | O157:H7 bovine strain | Our stocks, 40 |
| ZAP58 | O157:H7 human disease outbreak strain | Our stocks, 40 |
| ZAP193 | NCTC 12900; O157 stx Nalr | Our stocks |
| ZAP198 | O157 stx Nalr | Our stocks, 49 |
| ZAP1001 | ZAP1 sac-kan ΔlacZY | This study |
| ZAP1041 | ZAP41 sac-kan ΔlacZY | This study |
| ZAP1193 | ZAP193 sac-kan ΔlacZY | This study |
| ZAP1193-sep1-5 | ZAP1193 with sepL promoter regions from ZAP strains cloned in frame with lacZ and exchanged in place of the sac-kan cassette (sep-1, ZAP1; sep-2, ZAP21; sep-3, ZAP41; sep-4, ZAP46; sep-5, ZAP58) | This study |
| ZAP1193-espA1-6 | ZAP1193 with espA promoter regions from ZAP strains cloned in frame with lacZ and exchanged in place of the sac-kan cassette (espA1, ZAP1; espA2, ZAP21; espA3, ZAP41; espA4, ZAP46; espA5, ZAP58, espA6, ZAP193) | This study |