TABLE 4.
Miller assay results for plasmid-based LEE1-4 promoter fusions to β-galactosidase in high- and low-secretor E. coli O157 EspD backgrounds
| Plasmid | Avg no. of Miller units ± SD (n = 3) |
|
|---|---|---|
| ZAP1001a (high secretor) | ZAP1041a (low secretor) | |
| pAJR101 (LEE1::lacZ) | 7,429 ± 122 | 7,659 ± 187 |
| pAJR102 (LEE2::lacZ) | 2,364 ± 79 | 1,984 ± 97 |
| pAJR103 (LEE3::lacZ) | 113 ± 11 | 144 ± 45 |
| pJTPr-2 (LEE4::lacZ) | 353 ± 57 | 351 ± 42 |
a
Strains were grown in MEM-HEPES to an OD600 of 0.6, and Miller assays were carried out as described in Materials and Methods.