TABLE 5.
Miller assay results obtained with chromosomal sepL′ and espA′ translational fusions to lacZ in EHEC O157 ZAP1193
Promoter source | Avg no. of Miller units (± SD, n = 3) for indicated chromosomally integrated translational β-galactosidase fusions in ZAP1193a |
|
---|---|---|
sepL::lacZ | espA::lacZ | |
ZAP1 | 50 ± 2 | 3 ± 2 |
ZAP21 | 50 ± 3 | 4 ± 2 |
ZAP41 | 49 ± 1 | 2 ± 1 |
ZAP46 | 49 ± 2 | 2 ± 1 |
ZAP58 | 47 ± 2 | 4 ± 1 |
ZAP193 | NDb | 2 ± 1 |
No promoter | 2 ± 1 | 2 ± 1 |
Strains were grown in MEM-HEPES to an OD600 of 0.6, and Miller assays were carried out as described in Materials and Methods.
ND, not determined for this construct.