FIG. 4.

Antigens recognized by T cells from draining lymph nodes of Quackenbush mice immunized with pools of overlapping peptides derived from yMSP1₁₉. T-cell proliferative responses of mice immunized with peptide pool 1 (peptides 15 to 19 [P15 to P19]) (left) or with peptide pool 2 (peptides 20 to 24 [P20 to P24]) (right) are shown. The cells were plated in triplicate, and each symbol is the mean of an individual mouse's response to each peptide. The peptide and protein concentration used to stimulate the T cells was 30 μg/ml. Cells were incubated with purified protein derivative (PPD), concanavalin A (CONA), and Eagle minimum essential medium as controls.