Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1981 May;43(5):659–668. doi: 10.1038/bjc.1981.97

Measurement of Gross cell-surface antigen and p30 level in murine retrovirus-infected cell lines.

D Gerlier, S Gisselbrecht, B Guillemain, J F Doré
PMCID: PMC2010674  PMID: 7248150

Abstract

The level of Gross cell-surface antigen (GCSAa) expression at the surface of murine retrovirus-infected fibroblasts was determined by quantitative absorption of the anti-GCSAa activity of a serum produced in syngeneic W/Fu rats immunized against (C58NT)D lymphoma, and tested in a cytotoxicity assay against E male G2 lymphoma cells. While GCSAa was specifically expressed on Gross-type virus (G-MuLV)-induced lymphoma cells, and while G-MuLV and G-related MuLV induced a high level of GCSAa expression on murine fibroblasts, the Friend-Moloney-Rauscher (FMR) group viruses (FMR MuLV) and xenotropic isolates were also able to induce a high or intermediate level of GCSAa. Since GCSAa has been shown to be borne by glycosylated precursors of the viral nucleocapside (gp95gag and gp85gag), the amount of GCSAa expressed on these cells was compared to the level of cytoplasmic p30. In G- and G-related MuLV-infected cell lines, a significant relationship was found between the amount of GCSAa and the level of p30, whereas in FMR-MuLV or xenotropic virus-infected cells the amount of GCSAa varied independently of the p30 level. These results could explain the discrepancy in the specificity of expression of GCSAa in vivo and in vitro.

Full text

PDF
659

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnstein P., Levy J. A., Oshiro L. S., Price P. J., Suk W., Lennette E. H. Recovery of murine xenotropic type-C virus from C57L mice. J Natl Cancer Inst. 1974 Dec;53(6):1787–1792. [PubMed] [Google Scholar]
  2. Bassin R. H., Tuttle N., Fischinger P. J. Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. Int J Cancer. 1970 Jul 15;6(1):95–107. doi: 10.1002/ijc.2910060114. [DOI] [PubMed] [Google Scholar]
  3. Bauer H. Virion and tumor cell antigens of C-type rna tumor viruses. Adv Cancer Res. 1974;20:275–341. doi: 10.1016/s0065-230x(08)60112-8. [DOI] [PubMed] [Google Scholar]
  4. Buetti E., Diggelmann H. Murine leukemia virus proteins expressed on the surface of infected cells in culture. J Virol. 1980 Mar;33(3):936–944. doi: 10.1128/jvi.33.3.936-944.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dexter D. L. A function which describes quantitative adsorptions of antibodies by cell-surface antigens. Immunochemistry. 1976 Oct;13(10):801–806. doi: 10.1016/0019-2791(76)90179-8. [DOI] [PubMed] [Google Scholar]
  6. Edwards S. A., Fan H. gag-Related polyproteins of Moloney murine leukemia virus: evidence for independent synthesis of glycosylated and unglycosylated forms. J Virol. 1979 May;30(2):551–563. doi: 10.1128/jvi.30.2.551-563.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Evans L. H., Dresler S., Kabat D. Synthesis and glycosylation of polyprotein precursors to the internal core proteins of Friend murine leukemia virus. J Virol. 1977 Dec;24(3):865–874. doi: 10.1128/jvi.24.3.865-874.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fischinger P. J., O'Connor T. E. Productive infection and morphologic alteration of human cells by a modified sarcoma virus. J Natl Cancer Inst. 1970 Feb;44(2):429–438. [PubMed] [Google Scholar]
  9. GORER P. A. Studies in antibody response of mice to tumour inoculation. Br J Cancer. 1950 Dec;4(4):372–379. doi: 10.1038/bjc.1950.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Geering G., Old L. J., Boyse E. A. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J Exp Med. 1966 Oct 1;124(4):753–772. doi: 10.1084/jem.124.4.753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gerlier D., Guibout C., Dore J. F. Highly cytotoxic antisera obtained in W/Fu rats against a syngeneic Gross virus induced lymphoma. Eur J Cancer. 1977 Aug;13(8):855–859. doi: 10.1016/0014-2964(77)90141-4. [DOI] [PubMed] [Google Scholar]
  13. Gerlier D., Guillemain B., Doré J. F., Duplan J. F. Expression d'un antigène associé au virus de gross à la surface de cellules murines productrices d'un oncornavirus des radioleucémies de la souris C57BL/6. C R Acad Sci Hebd Seances Acad Sci D. 1977 Jun 20;284(23):2431–2434. [PubMed] [Google Scholar]
  14. Gisselbrecht S., Bassin R. H., Gerwin B. I., Rein A. Dual susceptibility of A 3T3 mouse cell line to infection by N- and B-tropic murine leukemia virus: apparent lack of expression of the FV-1 gene. Int J Cancer. 1974 Jul 15;14(1):106–113. doi: 10.1002/ijc.2910140113. [DOI] [PubMed] [Google Scholar]
  15. Gisselbrecht S., Pozo F., Debré P., Hurot M. A., Lacombe M. J., Levy J. P. Genetic control of sensitivity to Moloney-virus-induced leukemias in mice. I. Demonstration of multigenic control. Int J Cancer. 1978 May 15;21(5):626–634. doi: 10.1002/ijc.2910210513. [DOI] [PubMed] [Google Scholar]
  16. Hartley J. W., Rowe W. P. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology. 1975 May;65(1):128–134. doi: 10.1016/0042-6822(75)90013-6. [DOI] [PubMed] [Google Scholar]
  17. Hartley J. W., Rowe W. P., Huebner R. J. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J Virol. 1970 Feb;5(2):221–225. doi: 10.1128/jvi.5.2.221-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Herberman R. B. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J Natl Cancer Inst. 1972 Jan;48(1):265–271. [PubMed] [Google Scholar]
  19. Humphrey D., Tsukamoto-Adey A., Witte O. M., Fox R., Jerabek L., Weissman I. L. A serologic comparison of Moloney lymphoma cell surface and Moloney oncornavirus antigens. J Immunol. 1979 Jul;123(1):412–418. [PubMed] [Google Scholar]
  20. Jainchill J. L., Aaronson S. A., Todaro G. J. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J Virol. 1969 Nov;4(5):549–553. doi: 10.1128/jvi.4.5.549-553.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ledbetter J. A., Nowinski R. C., Eisenman R. N. Biosynthesis and metabolism of viral proteins expressed on the surface of murine leukemia virus-infected cells. Virology. 1978 Nov;91(1):116–129. doi: 10.1016/0042-6822(78)90360-4. [DOI] [PubMed] [Google Scholar]
  22. Ledbetter J., Nowinski R. C., Emery S. Viral proteins expressed on the surface of murine leukemia cells. J Virol. 1977 Apr;22(1):65–73. doi: 10.1128/jvi.22.1.65-73.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ledbetter J., Nowinski R. C. Identification of the Gross cell surface antigen associated with murine leukemia virus-infected cells. J Virol. 1977 Aug;23(2):315–322. doi: 10.1128/jvi.23.2.315-322.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Levy J. A., Pincus T. Demonstration of biological activity of a murine leukemia virus of New Zealand black mice. Science. 1970 Oct 16;170(3955):326–327. doi: 10.1126/science.170.3955.326. [DOI] [PubMed] [Google Scholar]
  25. Levy J. A. Xenotropic viruses: murine leukemia viruses associated with NIH Swiss, NZB, and other mouse strains. Science. 1973 Dec 14;182(4117):1151–1153. doi: 10.1126/science.182.4117.1151. [DOI] [PubMed] [Google Scholar]
  26. Levy J. P., Leclerc J. C., Varet B., Oppenheim E. Study of the antigenic specificity of Graffi leukemic cells. J Natl Cancer Inst. 1968 Sep;41(3):743–750. [PubMed] [Google Scholar]
  27. Lieber M., Sherr C., Potter M., Todaro G. Isolation of type-C viruses from the Asian feral mouse Mus musculus molossinus. Int J Cancer. 1975 Feb 15;15(2):211–220. doi: 10.1002/ijc.2910150206. [DOI] [PubMed] [Google Scholar]
  28. Mamoun R., Guillemain B., Astier T., Portail J. P., Legrand E., Duplan J. F. Production et analyse d'un complexe viral dérivé d'un virus des radioleucoses de la souris C57BL. Int J Cancer. 1978 Jul 15;22(1):98–105. doi: 10.1002/ijc.2910220117. [DOI] [PubMed] [Google Scholar]
  29. McCoy J. L., Fefer A., Glynn J. P. Comparative studies on the induction of transplantation resistance in BALB-c and C57BL-6 mice in three murine leukemia systems. Cancer Res. 1967 Oct;27(10):1743–1748. [PubMed] [Google Scholar]
  30. Nowinski R. C., Emery S., Ledbetter J. Identification of an FMR cell surface antigen associated with murine leukemia virus-infected cells. J Virol. 1978 Jun;26(3):805–812. doi: 10.1128/jvi.26.3.805-812.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. O'Donnell P. V., Stockert E. Induction of GIX antigen and gross cell surface antigen after infection by ecotropic and xenotropic murine leukemia viruses in vitro. J Virol. 1976 Dec;20(3):545–554. doi: 10.1128/jvi.20.3.545-554.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Old L. J., Boyse E. A., Stockert E. The G (Gross) leukemia antigen. Cancer Res. 1965 Jul;25(6):813–819. [PubMed] [Google Scholar]
  33. Reznikoff C. A., Brankow D. W., Heidelberger C. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 1973 Dec;33(12):3231–3238. [PubMed] [Google Scholar]
  34. Schneider J., Hunsmann G. Surface expression of murine leukemia virus structural polypeptides on host cells and the virion. Int J Cancer. 1978 Aug 15;22(2):204–213. doi: 10.1002/ijc.2910220215. [DOI] [PubMed] [Google Scholar]
  35. Schultz A. M., Rabin E. H., Oroszlan S. Post-translational modification of Rauscher leukemia virus precursor polyproteins encoded by the gag gene. J Virol. 1979 Apr;30(1):255–266. doi: 10.1128/jvi.30.1.255-266.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Schäfer W., Seifert E. Production of a potent complement-fixing murine leukemia virus-antiserum from the rabbit and its reactions with various types of tissue culture cells. Virology. 1968 Jun;35(2):323–328. doi: 10.1016/0042-6822(68)90273-0. [DOI] [PubMed] [Google Scholar]
  37. Snyder H. W., Jr, Stockert E., Fleissner E. Characterization of molecular species carrying gross cell surface antigen. J Virol. 1977 Aug;23(2):302–314. doi: 10.1128/jvi.23.2.302-314.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Stephenson J. R., Aaronson S. A. A genetic locus for inducibility of C-type in BALB-c cells: the effect of a nonlinked regulatory gene on detection of virus after chemical activation. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2798–2801. doi: 10.1073/pnas.69.10.2798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Todaro G. J., Arnstein P., Parks W. P., Lennette E. H., Huebner R. J. A type-C virus in human rhabdomyosarcoma cells after inoculation into NIH Swiss mice treated with antithymocyte serum. Proc Natl Acad Sci U S A. 1973 Mar;70(3):859–862. doi: 10.1073/pnas.70.3.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Tung J. S., Fleissner E. Amplified env and gag products on AKR cells. Origin from different murine leukemia virus genomes. J Exp Med. 1980 Apr 1;151(4):975–979. doi: 10.1084/jem.151.4.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Tung J. S., Pinter A., Fleissner E. Two species of type C viral core polyprotein on AKR mouse leukemia cells. J Virol. 1977 Aug;23(2):430–435. doi: 10.1128/jvi.23.2.430-435.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Wright B. S., O'Brien P. A., Shibley G. P., Mayyasi S. A., Lasfargues J. C. Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses. Cancer Res. 1967 Sep;27(9):1672–1677. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES