Figure 4.

Murine globin gene expression in hGATA(+) and hGATA(−) mice. Expression of murine βh1, ζ, ɛ, and α globin gene is measured by RNase protection. The protected fragment sizes are 181 bp for βh1 mRNA; 151 bp for ζ mRNA; 145 bp for ɛ mRNA; and 128 bp for mRNA. Notice that there is no difference in expression of ɛy, βh1, β^maj, or β^min mRNA between hGATA(+) and hGATA(−) littermates. The mouse ɛy probe detects two bands in some mice and only one in others because the mouse strains used have two alleles of the ɛy gene that are transcribed at different cap sites.