FIG. 5.

Expression of TLR2 and TLR4 mRNAs by mo-DC treated with meningococcal OM. Cells were left untreated or exposed to the optimal 1-μg/ml concentration of OM from the parent H44/76 strain (OM) or from the LPS-deficient mutant (pLAK-OM), or to pure meningococcal LPS, for 24 h. Results show the averages ± SEs from experiments with mo-DC from five donors, except for TLR2 with pLAK-OM treatment, where four donors were used. *, P < 0.05 (t test). #, one additional donor (data not shown) did not show a change in TLR2 mRNA expression following treatment.