TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli strain | ||
| CC118 | C. Manoil | |
| E. faecalis strains | ||
| OG1RF | Rifampin, fucidic acid resistant | 6 |
| OG1SSp | Streptomycin, spectinomycin resistant | 6 |
| INY3000 | Streptomycin, spectinomycin resistant, aggregation mutant | 33 |
| Plasmids | ||
| pMSP3535 | Nisin-inducible cloning shuttle vector | 2 |
| pMSP7517 | Nisin-inducible Asc10 in pMSP3535 | 12 |
| pMSP3607 | N-terminal RGD→RAD in prgB | This study |
| pMSP3608 | C-terminal RGD→RAD in prgB | This study |
| pMSP3609 | Double RGD→RAD in prgB | This study |
| pCWΩ258 | 31-amino-acid insertion at base 258 in prgB gene of pMSP7517 | 36 |
| pCWΩ438 | 31-amino-acid insertion at base 438 in prgB gene of pMSP7517 | 36 |
| pCWΩ468 | 31-amino-acid insertion at base 468 in prgB gene of pMSP7517 | 36 |
| pCWΩ1419 | 31-amino-acid insertion at base 1419 in prgB gene of pMSP7517 | 36 |
| pCWΩ1551 | 31-amino-acid insertion at base 1551 in prgB gene of pMSP7517 | 36 |
| pCWΩ1638 | 31-amino-acid insertion at base 1638 in prgB gene of pMSP7517 | 36 |
| pCWΩ2064 | 31-amino-acid insertion at base 2064 in prgB gene of pMSP7517 | 36 |
| pCWΩ2601 | 31-amino-acid insertion at base 2601 in prgB gene of pMSP7517 | 36 |
| pCWΩ2979 | 31-amino-acid insertion at base 2979 in prgB gene of pMSP7517 | 36 |
| pCWΩ3414 | 31-amino-acid insertion at base 3414 in prgB gene of pMSP7517 | 36 |