FIG. 1.

Multiphosphoprotein analysis of THP-1 cells infected with live and heat-killed M. bovis BCG. Each sample is represented as a phosphoprotein fingerprint. Accurate intensity values for each band are the accumulated signal obtained over a given scan time for each blot. These are shown as numerical values in Table 1. The data shown here represent protein bands visualized as a snapshot of the scan time and may therefore not represent exact band intensities. The three gels represent untreated THP-1 cells (A), THP-1 cells infected with live M. bovis BCG (B), and THP-1 cells infected with heat-killed M. bovis BCG (C). Each lane was probed with one or more antibodies. The proteins indicated by arrows are discussed in the text. Lanes 1 and 21 in each panel contained molecular size standards. Antibodies against the phosphorylated proteins were as follows: lanes 2, NR1 (S⁸⁹⁶); lanes 3, adducin (S⁷²⁴) and CDK1 (Y¹⁵); lanes 4, CREB (S¹³³); lanes 5, ERK1/2 (T²⁰²/Y²⁰⁴) (T¹⁸³/Y¹⁸⁵) and p70 S6K (T³⁸⁹); lanes 6, RSK (T³⁶⁰/S³⁶⁴), RAF1 (S²⁵⁹), and MEK1/2 (S²¹⁷/S²²¹); lanes 7, GSK3α (S²¹), GSK3β (S⁹), and PKB (T³⁰⁸); lanes 8, GSK3α (Y²⁷⁹/Y²¹⁶); lanes 9, PKR1 (T⁴⁵¹); lanes 10, PKCɛ (S⁷¹⁹); lanes 11, PKCα (S⁵⁶⁷); lanes 12, PKCα/β (T⁶³⁸) and SRC (Y⁵²⁹); lanes 13, PKCδ (T⁵⁰⁵); lanes 14, SAPK (T¹⁸³/Y¹⁸⁵); lanes 15, MSK 1/2 (S³⁷⁶) and JUN (S⁷³); lanes 16, JAK2 (Y¹⁰⁰⁷/Y¹⁰⁰⁸) and p38MAPK (T¹⁸⁰/Y¹⁸²); lanes 17, STAT1 (S⁷⁰¹); lanes 18, STAT3 (S⁷²⁷); lanes 19, RB (S⁷⁸⁰); lanes 20, RB (S⁸⁰⁷/S⁸¹¹).