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. 2003 Oct;71(10):5514–5522. doi: 10.1128/IAI.71.10.5514-5522.2003

FIG. 3.

FIG. 3.

Western analysis of adducin phosphorylation in THP-1 cells. (A) Adducin is phosphorylated in cells infected with BCG but not in cells infected with heat-killed bacteria. Panel 1, Western blot of THP-1 cells infected with live or heat-killed (HK) BCG for 24 h; panel 2, densitometry analysis of panel 1. (B) Time-dependent phosphorylation of adducin by M. bovis BCG infection. Panel 1, Western blot of THP-1 cells infected with BCG for 2, 12, or 24 h; panel 2, densitometry analysis of panel 1. (C) Adducin phosphorylation by purified ManLAM. Panel 1, Western blot of THP-1 cells treated with increasing concentrations of purified ManLAM; panel 2, densitometry analysis of panel 1. Error bars represent standard errors from at least three independent experiments. Equal loading of protein was verified by India ink staining.