TABLE 1.
Comparison of phosphorylated host proteins
| Protein
|
Epitope(s) | Signala
|
||||
|---|---|---|---|---|---|---|
| Full name | Abbreviation | Control | Fold change
|
|||
| Live avg | LAM | HK | ||||
| Alpha adducin | α-Adducin | S724 | 1 | 1.75b | 2.62 | 0.38 |
| Gamma adducin | γ-Adducin | S662 | 1 | 0.00 | 2.15 | NDc |
| Cyclic AMP response element binding protein | CREB | S133 | 1 | 0.00 | 1.00 | 0.86 |
| Cyclin-dependent kinase 1 (cdc2) | CDK1 | Y15 | 1 | 0.37 | 2.07 | 0.00 |
| Double-standard RNA-dependent protein kinase | PKR | T451 | 1 | 1.16 | 1.88 | 0.57 |
| Extracellular regulated kinase 1 | ERK1 | T202/Y204 | 0 | 0.51 | 0.00 | 1.01 |
| Extracellular regulated kinase 2 | ERK2 | T185/Y204 | 0 | 0.65 | 0.00 | 0.79 |
| Glycogen synthase kinase 3 alpha | GSK3α | Y279 | 1 | 1.29 | 1.44 | 0.82 |
| Glycogen synthase kinase 3 alpha | GSK3α | S21 | 1 | 0.92 | 1.79 | 0.36 |
| Glycogen synthase kinase 3 beta | GSK3β | Y216 | 1 | 1.57b | 1.52 | 0.65 |
| Glycogen synthase kinase 3 beta | GSK3β | S9 | 0 | 0.00 | 0.00 | 0.36 |
| Mitogen-activated protein kinase kinase 1/2 | MEK1/2 | S221/S225 | 1 | 1.85 | 1.69 | 0.39 |
| Mitogen-activated protein kinase kinase 3 | MEK3 | S189/T193 | 0 | 0.00 | 0.00 | ND |
| Mitogen-activated protein kinase kinase 6 | MEK6 | S207/T211 | 0 | 0.00 | 0.00 | ND |
| Mitogen- and stress-activated protein kinase 1/2 | MSK1/2 | S376 | 1 | 1.46 | 2.69 | 0.48 |
| Mitogen- and stress-activated protein kinase 1/2 | MSK1/2 | S376 | 1 | 2.71 | 4.01 | 0.33 |
| N-Methyl-d-aspartate glutamate receptor subunit 1 | NR1 | S896 | 1 | 1.93b | 1.24 | 0.91 |
| Oncogene JUN | JUN | S73 | 1 | 2.08b | 1.30 | 1.21 |
| Oncogene Raf 1 | RAF1 | S259 | 1 | 1.28 | 3.01 | 0.20 |
| Oncogene Raf 1 | RAF1 | S259 | 1 | 1.78 | 4.71 | 0.28 |
| Oncogene SRC | SRC | Y529 | 1 | 3.18 | 1.35 | 0.83 |
| Oncogene SRC | SRC | Y418 | 0 | 0.00 | 0.00 | ND |
| p38 alpha mitogen-activated protein kinase | p38MAPK | T180/Y182 | 1 | 4.91 | 1.10 | 0.74 |
| Protein kinase B alpha (Akt1) | PKBα | S473 | 0 | 0.00 | 0.00 | ND |
| Protein kinase B alpha (Akt1) | PKBα | T308 | 1 | 1.08 | 1.60 | 0.63 |
| Protein kinase C alpha | PKCα | S657 | 1 | 1.61 | 1.93 | 0.55 |
| Protein kinase C alpha/beta | PKCα/β | T638/641 | 1 | 1.69 | 2.82 | 0.51 |
| Protein kinase C delta | PKCδ | T505 | 1 | 1.28 | 2.59 | 0.43 |
| Protein kinase C epsilon | PKCε | S719 | 1 | 0.00b | 1.21 | 0.00 |
| Retinoblastoma 1 | RB | S780 | 1 | 0.82 | 1.85 | 0.64 |
| Retinoblastoma 1 | RB | S807/S811 | 1 | 0.35 | 2.05 | 0.54 |
| Ribosomal S6 kinase 1 | RSK1 | T360/S364 | 0 | 0.53 | 0.00 | 0.31 |
| S6 kinase p70 | p70 S6K | T389 | 1 | 2.40 | 3.93 | 0.57 |
| S6 kinase p70 | p70 S6K | T389 | 1 | 2.60 | 4.27 | 0.71 |
| Signal transducer and activator of transcription 1 | STAT1 | S701 | 1 | 2.05 | 3.02 | 0.80 |
| Signal transducer and activator of transcription 3 | STAT3 | S727 | 1 | 1.93 | 2.95 | 0.00 |
| Signal transducer and activator of transcription 5 | STAT5 | Y694 | 0 | 0.00 | 0.00 | ND |
| SMA- and MAD-related protein 1 | SMAD1 | S463/465 | 0 | 0.00 | 0.00 | ND |
| Stress-activated protein kinase (JNK) | SAPK | T183/Y185 | 1 | 2.55b | 3.07 | 0.68 |
| Stress-activated protein kinase (JNK) | SAPK | T183/Y185 | 1 | 3.36b | 1.85 | 0.80 |
The trace quantity of each protein band is measured by the area under its intensity profile curve and corrected for the individual scan times (recorded time before saturation occurs). Values for the control samples have been set to 1 or 0. A value 0 indicates that no immunoreactive signal was detected for this protein in either of the two screens. Live average is the average value, expressed as fold change, for the difference in phosphorylation between live infection and respective control samples for both screens. Values for LAM and heat-killed bacteria (HK) show the fold change relative to their respective control samples.
Proteins that showed a similar and greater-than-25% increase in phosphorylation for both screens.
ND, not determined.