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Avidity curves for the chimeric antibodies and the original mouse MAbs. (A) Results obtained with 184,F-12. (B) Results obtained with 151,F-9. (C) Results obtained with 208,D-5. The graphs show the influence of NH4SCN on the binding of the MAbs. The avidity index was the concentration of NH4SCN that resulted in a 50% reduction in absorbance. The curves show the nonlinear regression fit (Boltzman sigmoidal) obtained by using the Prism program supplied by GraphPad.
