TABLE 2.
Fold increase in gene expression per 100,000 CFU of S. pneumoniae relative to normal (noninoculated) controls
| Inflammatory mediator | S. pneumoniae phenotype variant | Fold increase in gene expression
|
|||
|---|---|---|---|---|---|
| Nonlavageda
|
Lavageda
|
||||
| 4 h | 24 h | 4 h | 24 h | ||
| IL-1α | Opaque | 12.1 | 0.9 | 14.1 | 0.8 |
| Transparent | 26.1 | 4.4 | 33.2 | 4.1 | |
| IL-1β | Opaque | 72.0 | 4.9 | 96.6 | 5.4 |
| Transparent | 264.2 | 18.8 | 138.0 | 20.6 | |
| IL-6 | Opaque | 112.6 | 3.7 | 100.1 | 4.2 |
| Transparent | 132.6 | 14.0 | 107.6 | 12.9 | |
| IL-10 | Opaque | 5.9 | 0.7 | 7.9 | 1.0 |
| Transparent | 14.3 | 2.5 | 15.1 | 3.7 | |
| iNOS | Opaque | 173.3 | 42.5 | 217.3 | 46.6 |
| Transparent | 489.0 | 123.0 | 513.4 | 94.8 | |
| TNF-α | Opaque | 17.4 | 0.7 | 13.9 | 0.6 |
| Transparent | 41.6 | 7.2 | 44.7 | 7.7 | |
a
Total RNA samples for the analysis of gene expression were prepared by in situ lysis of the middle ear epithelium either directly (nonlavaged) or following lavage to remove inflammatory cells at 4 and 24 h postinoculation with 2 × 105 CFU of opaque or transparent S. pneumoniae.