Skip to main content
. 2003 Oct;71(10):5541–5548. doi: 10.1128/IAI.71.10.5541-5548.2003

FIG. 5.

FIG. 5.

Lipid moieties of pneumococcal LTA are critical in TLR2 stimulation. CHO/CD14/TLR2 or CHO/CD14/TLR4 cells were treated with pneumococcal LTA, LTA-1 (monoacylated), LTA-0 (delipidated), or C-PS at the indicated concentrations for 16 h (A and B). Likewise, CHO/CD14/TLR2 was stimulated with pneumococcal LTA in the presence of LTA-0, C-PS, or anti-PC antibody (TEPC-15) at the indicated amounts for competition experiments (C, D, and E). The cells were stained with FITC-labeled anti-CD25 monoclonal antibody and subjected to flow cytometric analysis for the expression of the NF-κB-dependent reporter gene CD25.