FIG. 4.

Real-time PCR. (A to C) The cDNAs encoding periplasmic carbonic anhydrase (A), aliphatic amidase (B), and arginase (C) from typical experiments of the microarray analysis at pH 7.4 and 4.5 in the absence of urea were subjected to PCR amplification. The lines represent amplifications from cDNAs generated from the RNAs isolated at pH 7.4 and 4.5. (D) Similar amplification for the amidase cDNA resulting from temperature stress (42°C) compared to the cDNA generated from the RNA isolated from bacteria maintained at 37°C.