TABLE 1.
Primers used for RT-PCR analysis of murine cytokine mRNAs
| Target mRNA | Genbank accession no. | Primer sequences
|
Size of PCR amplicon (bp) | Refer- ence | |
|---|---|---|---|---|---|
| Sense | Antisense | ||||
| β-Actin | M12481 | GTGGGCCGCTCTAGGCACCA | CGGTTGGCCTTAGGGTTCAGGGGGG | 245 | 24 |
| IL-2 | NM_008366 | ACTTCAAGCTCCACTTCAAG | GAGTCAAATCCAGAACATGC | 256 | 27 |
| IL-4 | NM_021283 | CCAGCTAGTTGTCATCCTGCTCTT CTTTCTCG | CAGTGATGTGGACTTGGACTCATTCATGGTGC | 358 | 17 |
| IL-5 | NM_010558 | GACAAGCAATGAGACGATGA | GAACTCTTGCAGGTAATCCA | 235 | 27 |
| IL-10 | NM_010548 | GTGAAGACTTTCTTTCAAACAAAG | CTGCTCCACTGCCTTGCTCTTATT | 274 | 24 |
| IFN-γ | M28621 | AACGCTACACACTGCATCTT | GACTTCAAAGAGTCTGAGGT | 237 | 27 |
| TGF-β | BC013738 | TGGACCGCAACAACGCCATCTATG AGAAAACC | TGGAGCTGAAGCAATAGTTGGTATCCAGGGCT | 525 | 28 |