FIG. 2.

Proliferative responses of spleen cells from mice immunized with BLS. Mice (five per group) were immunized with rBLS in the absence (BLS-PBS) or the presence of BLS-Al, BLS-MPA, or BLS-IFA. Mice immunized with PBS were used as controls. Spleen cells from immunized mice (2 × 10⁵ cells/well) were stimulated with rBLS (0.5 μg/ml) for 5 days, and incorporation of [³H]thymidine was measured. The S.I. corresponds to the count per minute of stimulated cells divided by the count per minute of unstimulated cells. Results are expressed as the means ± standard errors of the means for five individual mice. Data are representative of three separate experiments. ★, significantly different from PBS-immunized group result (P < 0.05).