Table 1.
Affinity constants of synthetic oligosaccharides and one synthetic peptide for the anti-dGAS mAbs
| mAb | Affinity constant for hapten, 104 M−1
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mono | Di | Lin Tri | Bra Tri | Tetra 1 | Tetra 2 | Penta | Hexa 1 | Hexa 2 | CWPS* | DRPVPY-peptide | |
| SA-3 | 0.1 | 2.4 | 0.6 | 5.6 | 7.0 | 3.8 | 3.6 | 4.0 | 3.3 (5.71 ± 0.49)† | 3.6 | (160 ± 0.43)† |
| Strep 9 | 0.2 | 0.3 | <0.1 | 0.7 | 0.6 | 2.0 | 2.7 | 1.6 | 3.7 (3.09 ± 0.67)† | 10.4 | ND |
| HGAC 39 | ND | 0.4 | 0.1 | 0.9 | ND | 2.1 | 2.3 | ND | 1.7 | ND | ND |
| HGAC 47 | ND | 0.1 | <0.1 | 0.1 | ND | 0.1 | 1.3 | ND | 0.1 | ND | ND |
| HGAC 101 | ND | 0.2 | <0.1 | 0.2 | ND | <0.1 | 0.3 | ND | <0.1 | ND | ND |
Affinity constants are the inverse of the IC50 values (11) determined by competition ELISA against immobilized dGAS. The error in the values determined by ELISA is approximately 10%. ND, not determined.
*
The CWPS isolated and purified from GAS (12).
†
Data in parentheses were determined by titration microcalorimetry.