. 1997 Mar 18;94(6):2454–2459. doi: 10.1073/pnas.94.6.2454

Table 2.

The phage-displayed peptide sequences isolated by the anti-dGAS mAbs, SE155.4 and SYAJ/6

mAb	Library^*	Peptide sequence^†	ΔA × 1000
SA-3	X₆	DRPVPY	765
		KSPTPY	641
		ARPLWY	583
		VRPQVP	524
	fd-tet^‡	—	169
Strep 9	XCX₈CX	MCPPLYSPSACA	957
		ECNFLYPGFTCA	202
	X₈CX₈	YPYCGHALCPGLYADAS	1020
		VILPYDNNCALCLNLYP	644
		VIDAPTPNCAWPNGRRG	256
		MPPAGTGTCFLYALSCS	153
		ADLSPTPYCQPSTMHTN	144
		NEYINQDHCLLYAMLCP	38
	X₁₅CX	EIAPQGPSKCLLYAYCQ	13
	f88^‡	—	14
HGAC 39	X₁₅	ADAAPSPTPYLPRLS	643
		ATYRPVPAEFARKHL	425
		TITATDSPTPWPFER	244
	XCX₈CX	MCRPSPYNPPCT	112
	f88^‡	—	72
HGAC 47	XCX₈CX	MCRPGIPTHHCA	174
		HCSPGQRPGTCQ	165
		DCGNMLHAEVCR	149
		DCRPGVPLLSCP	115
	f88^‡	—	87
HGAC 101	XCX₆CX	SCISAACFCI	141
	X₆	KQLMAP	138
	f88^‡	—	29
SE155.4	X₆	NYPMDH	141
		MYPMSH	104
		YPMGHL	27
		IYPMPA	13
		QQYPMG	12
		QSTYPM	10
	X₁₅	EPYPMSEANYVRPMP	267
		YPMPASSDNAQWLLK	15
		DGTNAYPMNEDISVS	15
		HSTRNYSYLGSPYPM	13
		NYPMSGARIEPLLHA	13
		YAATEPRYMIPYPMP	13
		YPMGETCQRIRSCVW	11
	XCX₈CX	VCPAPYPAGTCA	11
	f88^‡	—	14
SYAJ/6	X₄CX₄CX₄	YTTQCGYGGCMNFE	938
		MGVICMNMECDRNM	799
		LHEYCNMETCPYNH	585
		QYPQCHNMDCKSIT	472
		PTHVCYNMECQGGD	275
		TPTNCYNMTCQNQP	187
	X₆	MDWNMH	993
	f88^‡	—	66

The listed peptides displayed on cpIII are preceded by the N-terminal sequence ADGA. In the sequence of the peptide library, X represents “randomized” amino acids and C stands for fixed cysteines.

^†

Sequences in boldface were chosen for further study; see Table 3. Consensus sequences are underlined.

^‡

Wild-type phage vector without a random peptide insert.