Table 3.
Immobilized antigen | ΔA × 1000 with mAb
|
||||||
---|---|---|---|---|---|---|---|
SA-3 | Strep 9 | HGAC 39 | HGAC 47 | HGAC 101 | SE155.4 | SYAJ/6 | |
DRPVPY-peptide | 300 | 28 | 21 | 20 | 24 | ND | ND |
Streptavidin* | 53 | 22 | 16 | 15 | 17 | ND | ND |
MCPPLYSPSACA | 92 | 446 | 40 | 44 | 25 | 10 | 33 |
YPYCGHALCPGLYADAS | 7 | 674 | 42 | 45 | 22 | 8 | 29 |
ADAAPSPTPYLPRLS | 47 | 14 | 726 | 62 | 31 | 12 | 35 |
MCRPGIPTHHCA | 6 | 13 | 44 | 148 | 29 | 11 | 33 |
DCGNMRQAEVCR | 7 | 12 | 41 | 160 | 26 | 10 | 32 |
SCISAACFCI | 6 | 12 | 80 | 120 | 51 | 12 | 36 |
KQLMAP | 6 | 12 | 95 | 121 | 43 | 11 | 34 |
NYPMDH | 6 | 12 | 58 | 78 | 37 | 112 | 36 |
EPYPMSEANYVRPMP | 6 | 11 | 56 | 74 | 32 | 145 | 35 |
MDWNMH | 6 | 16 | 57 | 69 | 34 | 13 | 689 |
MGVICMNMECDRNM | 6 | 12 | 47 | 54 | 28 | 10 | 283 |
YTTQCGYGGCMNFE | 8 | 14 | 55 | 55 | 32 | 12 | 483 |
f88† | 7 | 11 | 48 | 55 | 26 | 10 | 34 |
Numbers in boldface indicate that a phage clone bearing the listed sequence was isolated by the corresponding mAb (e.g., SA-3 isolated the clone bearing the DRPVPY sequence). Underlined numbers indicate significant crossreactivity with a mAb not used to select the clone. ND, not determined.
Streptavidin-coated wells used as a negative control for the DRPVPY-peptide-coated wells.
Wild-type phage vector without a random peptide insert.