Figure 2.

A TRAF3 mutant inhibits cell death by LTβR. The HT29.14S clones expressing TRAF3Δ1–367 were incubated in medium containing either recombinant cytokines (soluble LTα₁β₂ or TNF) or receptor-specific antibodies (purified goat anti-LTβR IgG or anti-Fas IgM, CH11). Cells were plated at 10⁴ cells per well in microtiter plates and cell viability was determined after 3 days by the MTT dye reduction assay. Each data point represent the mean ± SD of triplicate wells. The HT29.14S parental line (14S) and a pool of G418-resistant clones transfected with empty pCDNA3 plasmid (vec) were used as controls. The data shown was collected in one experiment. A summary of several determinations is shown in Table 1.