Figure 4.

Recruitment of TRAFΔ1–367 to the LTβR signaling complex. Cell lines were labeled for 3 hr with [³⁵S]methionine and [³⁵S]cysteine and then treated with (+) or without (−) LTα₁β₂ at 1 nM for 15 min. The cell lysates were subjected to immunoprecipitation with goat anti-LTβR and separation of proteins by SDS/PAGE. Radioactivity was detected by a PhosphorImager (pixel range 30–600). Immunoprecipitates from the indicated cell lines formed with preimmune goat serum (lanes 1, 3, 5, 7, 9, and 11) or goat anti-LTβR (lanes 2, 4, 6, 8, 10, and 12). The density of LTβR bands measured at lower sensitivity were equivalent (<1% variation) between the cell lines.