Table 1.

Effect of TRAF3Δ1–367 on ligand-induced cell death

Transfected HT29.14S clones	IC50 for ligand
	LTα1β2, pM	TNF, pM	Anti-Fas, ng/ml	Anti-LTβR, μg/ml
Empty vector	440 ± 350 (12)	20 ± 10 (9)	60 ± 58 (9)	1.2 ± 1 (9)
TRAF3Δ1–367	5200 ± 3000** (8)	200 ± 110* (8)	30 ± 30 (8)	9.5 ± 0.7** (8)

Concentration of LTα₁β₂ and TNF, anti-Fas, or goat anti-LTβR IgG required to induce 50% loss of cell viability (IC₅₀) as measured by MTT dye reduction assay. The IC₅₀ values are given as the mean ± SD as determined for a number (n) of clones transfected with empty vector or with TRAF3Δ1–367 expression vector. TRAF3Δ1–367 expression vector clones are those which expressed TRAF3Δ1–367 as determined by binding to LTβR-GST. The statistical significance of the difference in IC₅₀ between empty vector and TRAF3Δ1–367 clones was determined by the Student’s t test: ∗, P = 0.03; ∗∗, P < 0.002.