FIG. 8.

Binding and ingestion of opsonized sheep erythrocytes (EIgG) by J774A.1 macrophages. These macrophages were exposed for 1 h at 33°C to H. ducreyi 35000HP or to the lspA1 lspA2 mutant followed by 30 min of incubation at 37°C with EIgG. To measure ingestion (right-hand panels), extracellular erythrocytes were lysed with 0.2% saline. The samples were examined by light microscopy.