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Analysis of the expression of mce1 genes. (A) Schematic representation of the mce operons. The arrowheads indicate the position of the junction primers (J1 to J5). (B) Amplicons of RT-PCR with junction primers. Primer sets used are indicated above each lane. M, 100-bp ladder as a size marker; -ve, negative control, where mce1 primers were used in a PCR without an RT step. The RNA concentration was 300 ng per 20 μl of reaction mixture. The fast-moving band has the primer dimers.
