Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Analysis of expression profile of mce operons in exponential phase. Amplicons of RT-PCR with specific primers for mce1, mce2, mce3, and mce4 and RNA from 10-day-old cultures as the template were analyzed by electrophoresis; the operon-specific primers used are indicated above the lanes. M, 100-bp ladder. Equal concentrations of RNA (500 ng per 20 μl of reaction mixture) were used in all experiments; rpoB was used as a positive control. The fast-moving band has the primer dimers.
