TABLE 1.
Bacterial strains and plasmids used or identified in the study
| Species and strain or plasmida | Remarks | Source or referenceb |
|---|---|---|
| S. intermedius | ||
| 119-r | Wild-type strain, pNVH01 | This study, A |
| 139-r | Wild-type strain, pNVH01 | This study, B |
| 139-s | Closely related to 139-r, but plasmid free | This study, B |
| S. aureus | ||
| 120-r | Wild-type strain, pNVH01 | This study, A |
| 120-c | Isogenic to 120-r but cured for plasmid | This study, A |
| 140-r | Wild-type strain, pNVH01 | This study, B |
| 140-s | Isogenic to 140-r but plasmid free | This study, B |
| 538-r | Wild-type strain, pNVH01 | This study, C |
| 538-c | Isogenic to 538-r but cured for plasmid | This study, C |
| 184-r | Wild-type strain, pNVH01 | This study, D |
| RN4220 | Restriction-deficient, plasmid-free host strain | 12 |
| RN4220(pSK265) | RN4220 containing cloning vector pSK265 | 11 |
| RN4220(pSK265) smr | RN4220 containing smr gene and promoter | 8 |
| RN4220(pSK265) qacG | RN4220 containing qacG gene and promoter | 8 |
| RN4220(pSK265) qacH | RN4220 containing qacH gene and promoter | 7 |
| RN4220(pSK265) qacJ | RN4220 containing qacJ gene and promoter | This study |
| S. simulans 138-r | Wild-type strain, pNVH01 | This study, B |
| E. coli DH5α | Host strain for pUC18 | Life Technologies |
| Plasmids | ||
| pNVH01 | 2.650-kb; contains qacJ | This study |
| pUC18 SmaI/BAP | 2.686-kb cloning vector | Amersham Biosciences |
| pSK265 | 3.0 kb cloning vector | 11 |
| pSK265/qacJ | 3.6 kb with qacJ gene and promoter | This study |
a
r, QAC resistant; s, QAC sensitive; c, cured for pNVH01.
b
A, B, C, and D refer to the horses from which the strains were isolated.