TABLE 2.

Laboratory detection of hVISA

Laboratory (reference)	Detection methoda
	Simplified PAP on BHIA-V4	Simplified PAP on MHA	Broth microdilution (MIC)	Agar dilution (MIC)	Etest	PAP	Disk-agar	MicroScan	Resistant mutant emergence
Hiramatsu et al. (19)	I		CΨ
Franchi et al. (11)					I	C*
Ariza et al. (1)		X	X
Kantzanou et al. (23)	I♣		CΨ		C	C
Geisel et al. (14)	I		CΨ
Wong et al. (39)	I		CΨ		CΨ		I
Bierbaum et al. (2)	I		CΨ			C
Schmitz et al. (31)	X		X		X			X
Marchese et al. (26)	Iδ♣		CΨ			C
Kim et al. (24)	I		CΨ			C
Bobin-Dubreux et al. (3)		Iφ	C		C	C
Reverdy et al. (30)		Iφ		C	C	C	I
Trakulsomboon et al. (37)	Iδ		C			C			C
Wootton et al. (41)	X		X		X	X

^aDisk-agar, aztreonam disk on vancomycin salt; MicroScan, Microscan conventional gram-positive panels; I, initial screening test; C, confirmatory test; X, for the tests performed, did not distinguish between initial and confirmatory tests; *, PAP performed on MHA; Ψ, MIC determined on subclone recovered from initial screen; δ, 100 μl of starting cell suspension (instead of 10 μl) plated; φ, MHA with 2 μg of vancomycin per ml; ♣, McFarland standard of 1.0 (instead of 0.5) used.