TABLE 1.
Drug | Straina | Log killing (mean ± SEM)b
|
|||||||
---|---|---|---|---|---|---|---|---|---|
6-Mg
|
Neff
|
Taurine
|
NNA
|
||||||
4h | 6h | 4h | 6h | 4h | 6h | 4h | 6h | ||
PHMB (3 μg/ml) | Ros-91 | 4.0 ± 0.2 | 4.0 ± 0.2 | 1.7 ± 0.7 | 3.1 ± 0.2 | 0.4 ± 0.3 | 0.4 ± 0.3 | 2.6 ± 0.4 | 3.3 ± 0.2 |
Ros-02 | 0.9 ± 0.4 | 3.4 ± 0.6 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.8 ± 0.1 | 1.2 ± 0.3 | |
MAPD (20 μg /ml) | Ros-91 | 3.9 ± 0.1 | 3.9 ± 0.1 | 3.7 ± 0.1 | 3.7 ± 0.1 | 2.9 ± 0.4 | 3.8 ± 0.1 | 1.7 ± 0.2 | 2.8 ± 0.3 |
Ros-02 | 3.9 ± 0.1 | 3.9 ± 0.1 | 2.1 ± 0.2 | 4.5 ± 0.1 | 1.8 ± 0.0 | 2.9 ± 0.3 | 1.7 ± 0.2 | 2.7 ± 0.2 | |
MPS-1 (1 μg /ml) | Ros-91 | 3.6 ± 0.4 | 4.3 ± 0.1 | 2.8 ± 0.2 | 3.9 ± 0.6 | 2.8 ± 0.1 | 3.7 ± 0.4 | 1.6 ± 0.3 | 1.7 ± 0.3 |
Ros-02 | 3.0 ± 0.4 | 3.8 ± 0.1 | 1.9 ± 0.5 | 3.4 ± 0.2 | 1.2 ± 0.3 | 1.5 ± 0.3 | 0.9 ± 0.2 | 1.7 ± 0.4 | |
MPS-2 (1 μg /ml) | Ros-91 | 1.8 ± 0.4 | 3.7 ± 0.4 (2.9 ± 0.5)c | 0.8 ± 0.1 | 1.0 ± 0.0 (1.4 ± 0.2) | 2.4 ± 0.3 | 3.3 ± 0.4 | 0.4 ± 0.2 | 1.0 ± 0.2 (1.1 ± 0.3) |
Ros-02 | 1.7 ± 0.2 | 2.4 ± 0.1 | 1.2 ± 0.2 | 1.8 ± 0.3 | 1.1 ± 0.1 | 1.3 ± 0.0 | 0.2 ± 0.1 | 1.0 ± 0.1 | |
CHLX (30 μg /ml) | Ros-91 | 0.8 ± 0.1 | 1.0 ± 0.3 | 1.7 ± 0.3 | 1.8 ± 0.3 | 0.5 ± 0.1 | 0.8 ± 0.2 | 0.8 ± 0.1 | 1.5 ± 0.5 |
Ros-02 | 0.7 ± 0.2 | 1.2 ± 0.5 | 0.6 ± 0.4 | 1.1 ± 0.2 | 0.1 ± 0.1 | 0.4 ± 0.1 | 0.2 ± 0.3 | 0.2 ± 0.3 | |
Peroxide (3%) | Ros-91 | 2.9 ± 0.3 | 3.8 ± 0.1 | 2.4 ± 0.1 | 2.7 ± 0.1 | 2.1 ± 0.2 | 3.5 ± 0.5 | 2.5 ± 0.2 | 3.9 ± 0.5 |
Ros-02 | 3.3 ± 0.6 | 4.2 ± 0.2 | 1.7 ± 0.3 | 4.3 ± 0.1 | 2.5 ± 0.2 | 3.4 ± 0.6 | 0.9 ± 0.3 | 2.8 ± 0.5 | |
One-quarter-strength Ringer's (control) | Ros-91 | 0.1 ± 0.0 | 0.0 ± 0.0 | 0.2 ± 0.3 | 0.2 ± 0.1 | 0.1 ± 0.1 | 0.2 ± 0.2 | 0.2 ± 0.2 | 0.0 ± 0.1 |
Ros-02 | 0.2 ± 0.1 | 0.0 ± 0.2 | 0.3 ± 0.2 | 0.2 ± 0.4 | 0.0 ± 0.0 | 0.0 ± 0.1 | 0.1 ± 0.1 | 0.1 ± 0.2 |
A. polyphaga strain Ros-02 was cryopreserved shortly after isolation in 1991 and was recovered for this study; strain Ros-91 has been in continuous axenic culture since that time.
The experiments were conducted in triplicate.
The values in parentheses are the log killings obtained with additional batches of Ros-91 cysts tested on a separate occasion.