Figure 1.

Diagram of transgene constructs and expression of the transgenes. (A) Schematic structures of transgene constructs. Functional elements include the enhancer of the cytomegalovirus (open box), the promoter of the chicken β-actin (diagonally striped box), the coding sequence of the truncated or WT mouse gp130 cDNA including the ATG translation initiation codon and the TGA translation stop codon (black box), and rabbit β-globulin poly A (shaded box). The WT and truncated form of gp130 contain full (277 aa) or the membrane-proximal 63-aa part of the cytoplasmic region of gp130. The truncated form of gp130 did not have the membrane distal part containing box3. (B) Expression of truncated or WT gp130 in splenocytes. Nonidet P-40 lysates from splenocytes were immunoprecipitated with anti-mgp130 mAb (RX435) and immunoblotted with rabbit anti-mouse gp130 polyclonal Ab. NC, normal control mice; WT, transgenic mice expressing wild-type gp130; DN, transgenic mice expressing the truncated form of gp130.