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Impaired tyrosine phosphorylation of gp130 and STAT3 in transgenic mice expressing a truncated form of gp130. Splenocytes (1 × 107 cells) were stimulated with IL-6 (100 ng/ml) plus soluble form of IL-6R (200 ng/ml) for 10 min, and solubilized in Nonidet P-40 lysis buffer as described. The lysates were immunoprecipitated with RX435 or rabbit anti-STAT3 serum, then immunoblotted with antiphosphotyrosine (4G10).
