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Development of lymphocytes in DN transgenic mice. (A) Thymocytes from 4-week-old mice were stained with FITC-anti-CD8 and PE-anti-CD4. (B) Splenocytes freshly prepared from 6-week-old mice were stained with the following: FITC-anti-B220 and PE-conjugated anti-Thy-1; and FITC-anti-B220 and biotinylated anti-IgM + PE-conjugated avidin.
