Figure 4.
Impairment of IL-6 effects on lymphocytes from the transgenic mice expressing a dominant negative form of gp130. (A) Impairment of IL-6-induced antibody production in B cells from DN transgenic mice. Three days after immunization with SRBCs, spleen cells were cultured with SRBCs in the absence or presence of IL-6 for 3 days. SRBC-specific direct plaque-forming cell assay were counted using spleen cell suspensions as the source of antibody-secreting cells. Representative results of three separate experiments are shown. (B) Impaired costimulant effects of IL-6 on thymocytes from DN transgenic mice. Thymocytes from 4-week-old normal, WT, and DN mice were incubated at a density of 5 × 105 cells per well in the presence of PHA at 5 μg/ml for 3 days. The cultures were pulsed with [3H]thymidine during the last 16 hr of incubation.