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Antibody responses to DNP-OVA. WT (○) and DN (•) transgenic mice (6 weeks old) were injected i.p. with DNP-OVA in complete Freund’s adjuvant to induce IgM, IgG, and IgA responses; boosted 21 days later; then bled at indicated times. Levels of anti-DNP antibodies were determined using isotype-specific ELISA. All plots were means of duplicate data. We defined 1/1000 of titers against DNP of WT-sera from 28 days after immunization as arbitrary units.
