TABLE 1.
Primers used in the PCR and RT-PCR analysis of M. smegmatis
| Primer | Sequence (5′→3′) | Target |
|---|---|---|
| MSX-1 | ACGAGCTCGGCCAGAACTTCCTGT | erm |
| MSX-2 | CGTCTTCGCGAGGTCCTGACACAA | folD |
| MSX-3 | GGTGAGCGGGGCAGTGGGTAG | erm |
| MSX-4 | CGGCGGATCTGCGTCAGACACT | erm |
| MSX-5 | GGTCACCGCGGACATGGTCAAG | folD-Rv3355 |
| MSX-6 | ACCGACAGCCGCGATGATGATG | folD |
| MS23-1 | CGAATGGCGTAACGACTTCTCA | 23S rRNA |
| MS23-2 | GCGACACCCCTTCCACTCAG | 23S rRNA |
| MSX-4acca | ACCTCCGGACGGCGGATCTGCGTCAGACACT | erm |
| MSX-5bspa | CGATCGATGGTCACCGCGGACATGGTCAAG | erm |
| MSpgaE-1 | CGGTCGGGGATGCTGACAT | pgaE |
| KN10_U2 | CGCAGCACCACCGAGAA | pYUB854 |
| KN10_L1 | AGGCAACTATGGATGAACGAAAT | pYUB854 |
| SACUP-2nota | AAGGCGGCCGCTTGCAATCCAAACGAGAGTCTA | pK19mobsacb |
| SACDN | AATAGGATATCGGCATTTTCTTTTG | pK19mobsacb |
a
Underlined bases indicate restriction sites (acc, AccIII; bsp, Bsp106I; not, NotI) used to clone the amplimers into the expression vector pMV261.