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. 1983 Apr;47(4):497–502. doi: 10.1038/bjc.1983.79

A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis.

S A Engelholm, M Spang-Thomsen, L L Vindeløv
PMCID: PMC2011338  PMID: 6849794

Abstract

A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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