TABLE 2.
Characteristics of 13 Ureaplasma sp. and one M. hominis clinical isolates
| Strain | MIC (μg/ml)a
|
Amino acid change in QRDRb
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NOR | PEF | OFX | CIP | SPX | LVX | MXF | GEM | GyrA position:
|
ParC position:
|
ParE position 469 (462) | |||||
| 100 (83) | 112 (95) | 83 (80) | 87 (84) | 125 (123) | 136 (134) | ||||||||||
| Reference strain U. parvum | 32 | 2 | 1 | 1 | 0.25 | 0.5 | 0.25 | 0.25 | Gln | Asp | Ser | Glu | Ala | Ala | Ala |
| Clinical strains | |||||||||||||||
| UUe1 | 16 | 2 | 2 | 2 | 0.25 | 0.25 | 0.25 | 0.25 | —c | — | — | — | — | — | — |
| UUe2 | >128 | 64 | 8 | 8 | 2 | 2 | 1 | 0.5 | — | — | Leu | — | — | — | — |
| UUb, -d, -f1, and -g1 | 64 | 4-16 | 4-8 | 4-16 | 0.5 | 0.5-1 | 0.5-1 | 0.25 | — | Glu | — | — | Thr | Thr | — |
| UUf2 and -g2 to -g5 | 128 | 32-64 | 4-8 | 16 | 2-4 | 4 | 1 | 1 | — | Glu | Leu | — | Thr | Thr | — |
| UUc | >128 | 32 | 8 | 8 | 2 | 2 | 1 | 0.25 | — | Glu | — | Lys | Thr | Thr | — |
| UUad | >128 | 64 | 64 | 64 | 16 | 16 | 4 | 2 | Arg | Glu | — | — | Thr | Thr | — |
| M. hominise | |||||||||||||||
| PG21 | 8 | 2 | 0.5 | 1 | 0.03 | 0.5 | 0.06 | 0.06 | Ser | Asp | Ser | Glu | Ser | Lys | Ala |
| MHe | 64 | 32 | 16 | 8 | 4 | 16 | 4 | 2 | Trp | — | Ile | — | — | Arg | Val |
a
NOR, norfloxacin; PEF, pefloxacin; OFX, ofloxacin; CIP, ciprofloxacin; SPX, sparfloxacin; LVX, levofloxacin; MXF, moxifloxacin; GEM, gemifloxacin.
b
The number in parentheses is the number of the corresponding codon in E. coli for Ureaplasma spp. The QRDRs of gyrA, gyrB, parC, and parE were sequenced for all clinical isolates.
c
Identical to the reference strain.
d
From reference 5.
e
E. coli positions GyrA 83 and 95, ParC 80, 84, 123, and 134, and ParE 462 correspond to M. hominis coordinates GyrA 153 and 165, ParC 91 and 95, and ParE 468.