TABLE 1.
Oligonucleotides used for PCR and DNA sequence determination
| Gene | Primer name | Oligonucleotide sequence (5′ to 3′) | Amplified regiona | Sequenced regiona |
|---|---|---|---|---|
| gyrA | GyrAF21 | GAA CTC ACCC TTC CAG ATC CA | −248 to 734 | |
| GyrAR1000 | GAG CGC GGA TAT ACA CCT T | |||
| GyrAFQ322 | GAG CTC CTA TCT GGA TTA T | 135 to 667 | ||
| gyrB | GyrBF891 | GGA CAA AGA AGG CTA CAG CA | 891 to 1770 | |
| GyrBR1770 | CGT CGC GTT GTA CTC AGA TA | |||
| GyrBFQ1165 | GTG AAA TGA CCC GCC GTA AA | 1215 to 1770 | ||
| parC | ParCF43 | AGC GCC TTG CGT ACA TGA AT | 17 to 981 | |
| ParCR981 | GTG GTA GCG AAG AGG TGG TT | |||
| ParCFQ107 | GAC CGT GCG TTG CCG TTT AT | 142 to 766 | ||
| ParE | ParEF853 | GAC CGA AAG CTA CGT CAA CC | 788 to 1746 | |
| ParER1810 | GTT CGG ATC AAG CGT GGT TT | |||
| ParEFQ1126 | GCC CAG CGC CGT ATG CGT GC | 1171 to 1734 | ||
| marR | MarORF1139 | GCC AGG CCA AGA AAT AAC GC | −307 to +130 | |
| MarORR2011 | GAG TAA CCC GAA CGC TCT GA | |||
| MarORFQ1236 | GGT GGT TGT TAT CCT GTG TA | −151 to stop | ||
| MarORRQ1963 | GGT TGT CCT CGA TCC AGT C | Start to +10 | ||
| acrR | AcrR8900F | ACT GTT ACT ACG CCA ACG | −275 to +65 | |
| AcrR9934R | CTG AAC CTG AAG AAC GAC CTG | |||
| AcrRQ9021F | GGC ATC TGC TGG CCA CCT T | −150 to 315 | ||
| AcrRQ9887R | GAC GCA GTG AAC CAG AA | 135 to +28 |
a
Nucleotide 1 is the first nucleotide of the start codon. A minus sign refers to nucleotides upstream of the start codon, and a plus sign refers to nucleotides downstream of the termination codon.