TABLE 3.

Putative fluoroquinolone resistance mutations, organic solvent tolerance, and mutation rate

Strain	Resistance mutation					OSTa	μb	No. of RAM(s)c
	gyrA	parC	parE	marOR	acrR
C1205	S83L	S80R			F24L	+	18	4
	D87N
C1202	S83L	S80I			T5N	+	7	4
	D87Y
C1171	S83L	S80I		Q110 stop	162F	+	13	4
	D87H				IS10 at aad 106
					T → A at nt −10
C1181	S83L	S80I		K62R	T213I	−	2	3
	D87N				N214T
					E215A
C1203	S83L	S80I		K62R	ΔA at aa 80	+	45	4
	D87N				T213I
C4	S83L				+2 nt(GC) at aa 206	+	104	2
C1168	S83L	S80I	S458T	ΔG at aa 52	I62F	+	16	5
	D87N			A52R	E215A
				G/S103N
C1198	S83L	S80I	I444F		A212S	+	25	5
	D87N				T213I
C1163	S83L	S80I				−	11	3
	D87N
C1162	S83L	S80I		L78M	M201R	+	12	4
	D87G
C1173	S83L	S80I			E215A	−	10	3
	D87N
C1172	S83L	S80I		L78M	M201R	+	7	4
	D87G
C1204	S83L	S80I				-	9	4
	D87N	E84G
C1179	S83L				E215A	+	19	3
	D87N				S25P
C1188	S83L		I529L		E215A	−	2	3
	D87Y
C1186	S83L	S80I		Δ5 nt (−68 to 72)	Insertion of IS1 at aa 9	+	17	4
	D87N			C47G
C1176	S83L	S80I			A212S	+	6	4
	D87N				T213I
					N214T
					E215A
C1184	S83L		I529L		E215A	−	4	3
	D87Y
C1212	S83L	S80I			T213I	−	11	3
	D87N
C1199	S83L	S80I				+	14	3
	D87N
C1208	S83L	E84K				−	16	3
	D87N
C1192	S83L	S80I				−	4	3
	D87N
C1195	S83L	E84K			E215A	−	69	2
C139	S83L	S80I			E215A	−	3	2
C1200	S83L	S80I		D76G		+	36	4
	D87N							/PICK>
C1197	S83L	S80I			C → A at nt −25	−	3	3
	D87N				T213I
					N214T
					E215A
C1177	S83L	S80I			E215A	−	35	3
	D87G
C1191	S83L	S80I			K146T	+	6	4
	D87Y				IS5 at aa 73
C1211	S83L	S80I			T213I	−e	3	3
	D87N
C1180	S83L			V79I		−	4	2
C107	D87Y					+	12	1
C10	S83L				4-nt duplication (GATT) at aa 111-12	+	9	2
C1166	S83L				E215A	+	9	1
					T → A at nt −10
C142	S83L				E215A	−	10	1
C77	S83L					−	1	1
C128	S83L					−	4	1
C47				+17 nt (duplication) (aa 26-31)f		+	8	1
C2	D87G					−	2	1
C136			D475E		I16F	+	5	2
					V125I
C6						−e	1	0
C90	A196E					−	1	1
C50						−	6	0
C9						−e	69	0
C97					E215A	−	4	0
C120					E215A	−	3	0
C133					E215A	−	4	0
C149						−	3	0
C88						−	2	0
C151						−	2	0
C62						−	4	0
C25				K62R	T213I	−	7	0
					N214T
C150					P155L	+	2	1
C124					E215A	−	14	0
					N69H
Nu14						−	1	0

^aOST, organic solvent tolerance.

^bμ, mutation rate relative to that of Nu14 (5 × 10⁻⁹ per cell per generation for rifampin resistance), which was set equal to 1.

^cRAM(s), resistance-associated mutation(s). The number of mutations per strains included in the count (for rationale, see Results), in which only one mutation from marR and acrR is included.

^daa, amino acid(s).

^eStrains C1211, C6, and C9 are sensitive to hexane.

^fThe duplicated sequence (amino acids 26 to 31) in strain C47 is GAT CGC CTG CTT AAC GA] [GTT CGC CTG CTT AAC GA.