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. 2003 Oct;47(10):3296–3304. doi: 10.1128/AAC.47.10.3296-3304.2003

FIG. 5.

FIG. 5.

ODN binding to aac(6′)-Ib mRNA. Various concentrations of mRNA (100, 50, or 10 nM) were incubated with 5′-end-labeled ODNs (10 nM) as described in Materials and Methods and analyzed in native polyacrylamide gels. A reaction without mRNA was also performed (the results are indicated in the rightmost lanes of the gels). The upper bands are ODNs bound to mRNA. The ODNs used are indicated below the gels (the numbers correspond to those shown in Fig. 2). ODN S, control mixture containing an ODN with a sense sequence.