TABLE 1.
Bacterial strains and plasmids used in the study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| P. aeruginosa | ||
| K767 | PAO1 wild type | R. Hancock (University of British Columbia) |
| K258 | Clinical isolate | D. Speert (University of British Columbia) |
| K260 | Clinical isolate | D. Speert (University of British Columbia) |
| K2152 to K2156 | Clinical isolates | L. Tomalty (Kingston General Hospital) |
| K2157 to K2163 | Clinical isolates | D. Livermore (Antibiotic Resistance Monitoring and Reference Laboratory) |
| K1525 | K767 ΔmexXY | 3 |
| K2164 | K2152 ΔmexXY | Present study |
| K2165 | K2153 ΔmexXY | Present study |
| K2166 | K2155 ΔmexXY | Present study |
| K2167 | K2156 ΔmexXY | Present study |
| K2168 | K2160 ΔmexXY | Present study |
| K2169 | K2161 ΔmexXY | Present study |
| K2170 | K2162 ΔmexXY | Present study |
| K2171 | K258 ΔmexXY | Present study |
| K2172 | K2163 ΔmexXY | Present study |
| E. coli S17-1 | Donor strain used to promote transfer of pEX18Tc derivatives into P. aeruginosa; thi pro hsdR recA Tra+ | 27 |
| Plasmids | ||
| pEX18Tc | Gene replacement vector; sacB Tcr | 5 |
| pCSV05-01 | pEX18Tc::ΔmexXY | 3 |