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Thermal denaturation of empty and peptide-loaded I-Ek heterodimers monitored by CD at pH 7.0 (A) and pH 5.0 (B). Protein concentrations were 40–60 μg/ml. (C and D) Far-UV CD scans of native, unfolded, and renatured empty (C) and peptide-filled (D) I-Ek at neutral and mildly acidic pH.
